ExcludeMRSA Preventing transmission of MRSA from livestock to humans through competitive exclusion
ExcludeMRSA investigated reducing farmers' exposure to LA-MRSA (Life Stock Associated Methicillin Resistant Staphylococcus aureus) by treating piglets with a microflora that competes with this pathogen.
Goal
The objectives include identifying bacterial species through microbiome and competition studies, studying the efficacy of a nasal microflora on piglets under field conditions, and estimating risk reduction in MRSA transmission to humans.
Methods
The researchers analysed piglet nasal microbiomes, identifying species negatively correlated with MRSA and collected samples from 256 piglets across 3 countries for a strain biobank. Microbiome analysis revealed age and farm effects on the microbiome and they identified competing strains for pathogen reduction. A cocktail of strains was then applied to litters in Ireland and the Netherlands, with ongoing comparisons between treated and placebo groups.
Collaboration partners
The researchers collaborated with the ABR project MRSA-PREVENT (Dutch only).
Results
The study compared methods for measuring airborne MRSA on pig farms, suggesting the use of electrostatic dust fall collectors for quantifying MRSA levels. Results were published in scientific journals, contributing to the One Health approach for preventing AMR infections in agricultural settings.
Products
Author: Shriram Patel # 1, Abel A Vlasblom # 2, Koen M Verstappen 2, Aldert L Zomer 2, Ad C Fluit 3, Malbert R C Rogers 3, Jaap A Wagenaar 2 4, Marcus J Claesson 1, Birgitta Duim 2
Magazine: mSystems
Link: https://journals.asm.org/doi/10.1128/msystems.00152-21?url_ver=Z39.88-2003&rfr_id=ori%3Arid%3Acrossref.org&rfr_dat=cr_pub++0pubmed
Magazine: Ruth Rattigan 1, Lukasz Wajda 1, Abel A Vlasblom 2, Alan Wolfe 3, Aldert L Zomer 2, Birgitta Duim 2, Jaap A Wagenaar 2, Peadar G Lawlor 1
Start- and endpage: 3442-
Link: https://www.mdpi.com/2076-2615/13/22/3442
Author: Abel A. Vlasblom, Shriram Patel, Peadar G. Lawlor, Marcus J. Claesson, Daniel Crespo-Piazuelo, Julia Eckenberger, Chloe E. Huseyin, Christian Elend, Jaap A. Wagenaar, Aldert L. Zomer, Birgitta Duim
Magazine: biorxiv
Link: https://www.biorxiv.org/content/10.1101/2024.06.11.598526v1
Author: Abel A Vlasblom 1, Birgitta Duim 1 2, Shriram Patel 3 4, Roosmarijn E C Luiken 1, Daniel Crespo-Piazuelo 5, Julia Eckenberger 3, Chloe E Huseyin 3, Peadar G Lawlor 5, Christian Elend 6, Jaap A Wagenaar 1 2 7, Marcus J Claesson 3, Aldert L Zomer 1 2
Magazine: mSystems
Start- and endpage: 62624-
Link: https://journals.asm.org/doi/10.1128/msystems.00626-24?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%20%200pubmed
Author: Anne E Rittscher 1, Abel A Vlasblom 2, Birgitta Duim 2, Peter Scherpenisse 1, Isabella J van Schothorst 1, Inge M Wouters 1, Liese Van Gompel 1, Lidwien A M Smit 1
Magazine: Ann Work Expo Health
Start- and endpage: 1004-1010
Link: https://pmc.ncbi.nlm.nih.gov/articles/PMC10516621/