Generation and characterization of conditionally immortalized cardiomyocytes: A new and versatile cell platform for cardiac disease modelling, drug screening and regenerative medicine
Ronde 2016 Module Toepassen van Innovaties:
Fully differentiated human cardiomyocytes represent excellent alternatives to animals for developing models of cardiac disease, drug testing and identification of therapeutic targets. Thus far, the widespread use of human cardiomyocytes in biomedical research has been hampered by the difficulty to generate large, homogenous populations of these cells. This is mainly due to a shortage of human heart tissue and the inability of human cardiomyocytes to multiply themselves. Derivation of cardiomyocytes from human pluripotent stem cells could potentially solve these problems but is costly, laborious and, until now, has yielded relatively small-sized, immature and heterogeneous cell populations. We recently developed a robust and versatile alternative method to generate large numbers of fully differentiated cells from small tissues samples, which will be used in this study for producing lines of human cardiomyocytes to replace animals in applied and fundamental cardiac research.
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Titel: Transcriptome analysis of conditionally immortalized atrial myocytes: identification of a novel atrium-enriched protein involved in sarcomere assembly and maintenance
Auteur: P R R Van Gorp, J Zhang, J Liu, R Tsonaka, H Mei, S O Dekker, C I Bart, M J Schalij, T De Coster, D A Atsma, D A Pijnappels, A A F De Vries
Link: https://doi.org/10.1093/eurheartj/ehab724.3181
Auteur: P R R Van Gorp, J Zhang, J Liu, R Tsonaka, H Mei, S O Dekker, C I Bart, M J Schalij, T De Coster, D A Atsma, D A Pijnappels, A A F De Vries
Link: https://doi.org/10.1093/eurheartj/ehab724.3181
Titel: Conditionally immortalized atrial myocytes as potential cell source for myocardial regeneration
Auteur: Jia Liu, Linda Volkers, Masaya Watanabe, Annemarie Kip, Cindy I. Bart, Marc C. Engels, Zeinab Neshati, Wanchana Jangsangthong, Guangqian Zhou, Martin J. Schalij, Daniël A. Pijnappels, and Antoine A.F. de Vries
Auteur: Jia Liu, Linda Volkers, Masaya Watanabe, Annemarie Kip, Cindy I. Bart, Marc C. Engels, Zeinab Neshati, Wanchana Jangsangthong, Guangqian Zhou, Martin J. Schalij, Daniël A. Pijnappels, and Antoine A.F. de Vries
Titel: Identification of novel cardiomyogenic factors by transcriptome analysis of conditionally immortalized atrial myocytes
Auteur: P. van Gorp
Auteur: P. van Gorp
Titel: Massive expansion of native human atrial cardiomyocytes by immortogenetics for multiscale arrhythmia research
Auteur: N. Harlaar, L. Volkers, J. Liu, P.R. Van Gorp, A.A. Ramkisoensing, T.J. Van Brakel, D.A. Pijnappels, A.A.F. De Vries
Auteur: N. Harlaar, L. Volkers, J. Liu, P.R. Van Gorp, A.A. Ramkisoensing, T.J. Van Brakel, D.A. Pijnappels, A.A.F. De Vries
Titel: Massive expansion of native human atrial cardiomyocytes by immortogenetics
Auteur: N. Harlaar, L. Volkers, J. Liu, P.R. Van Gorp, A.A. Ramkisoensing, T.J. Van Brakel, D.A. Pijnappels, A.A.F. De Vries
Auteur: N. Harlaar, L. Volkers, J. Liu, P.R. Van Gorp, A.A. Ramkisoensing, T.J. Van Brakel, D.A. Pijnappels, A.A.F. De Vries
Titel: Molecular and functional mapping of cardiac electrophysiological differentiation
Auteur: Jia Liu, Bram Akerboom, Roula Tsonaka, Hailiang Mei, Martin J. Schalij, Daniël A. Pijnappels, Antoine A.F. de Vries
Auteur: Jia Liu, Bram Akerboom, Roula Tsonaka, Hailiang Mei, Martin J. Schalij, Daniël A. Pijnappels, Antoine A.F. de Vries
Titel: Massive expansion of native human atrial cardiomyocytes by immortogenetics
Auteur: N. Harlaar, L. Volkers, J. Liu, P.R. Van Gorp, A.A. Ramkisoensing, T.J. Van Brakel, D.A. Pijnappels, A.A.F. De Vries
Auteur: N. Harlaar, L. Volkers, J. Liu, P.R. Van Gorp, A.A. Ramkisoensing, T.J. Van Brakel, D.A. Pijnappels, A.A.F. De Vries
Titel: Massive expansion of native human atrial cardiomyocytes by immortogenetics for multiscale arrhythmia research
Auteur: N. Harlaar, L. Volkers, J. Liu, P.R.R. van Gorp, A.A. Ramkisoensing, M.J. Schalij, R.J.M. Klautz, T.J. van Brakel, D.A. Pijnappels, A.A.F. de Vries
Auteur: N. Harlaar, L. Volkers, J. Liu, P.R.R. van Gorp, A.A. Ramkisoensing, M.J. Schalij, R.J.M. Klautz, T.J. van Brakel, D.A. Pijnappels, A.A.F. de Vries
Titel: Transcriptome analysis of conditionally immortalized atrial myocytes: identification of a novel atrium-enriched protein involved in sarcomere assembly and maintenance
Auteur: P. van Gorp
Link: https://esc365.escardio.org/presentation/234535
Auteur: P. van Gorp
Link: https://esc365.escardio.org/presentation/234535
Titel: Generation and characterization of iAM-1, a versatile new line of conditionally immortalized atrial myocytes with preserved cardiomyogenic differentiation capacity.
Auteur: Jia Liu, Masaya Watanabe, Wanchana Jangsangthong, Annemarie Kip, Cindy I. Bart, Marc C. Engels, Zeinab Neshati, Linda Volkers, Guangqian Zhou, Martin J. Schalij, Daniël A. Pijnappels, Antoine A.F. de Vries
Auteur: Jia Liu, Masaya Watanabe, Wanchana Jangsangthong, Annemarie Kip, Cindy I. Bart, Marc C. Engels, Zeinab Neshati, Linda Volkers, Guangqian Zhou, Martin J. Schalij, Daniël A. Pijnappels, Antoine A.F. de Vries
Titel: Transcriptome analysis of conditionally immortalized atrial myocytes: identification of Sbk2, a novel atrium-enriched protein involved in sarcomere assembly and maintenance
Auteur: P.R.R. van Gorp
Auteur: P.R.R. van Gorp
Titel: Cellular models for fundamental and applied biomedical research
Auteur: Liu, Jia
Link: https://openaccess.leidenuniv.nl/handle/1887/67296
Auteur: Liu, Jia
Link: https://openaccess.leidenuniv.nl/handle/1887/67296
Titel: Conditional immortalization of human cardiomyocytes for translational in vitro modeling of cardiovascular disease
Auteur: Harlaar N, Pijnappels DA, de Vries AAF
Magazine: Cardiovascular Research
Auteur: Harlaar N, Pijnappels DA, de Vries AAF
Magazine: Cardiovascular Research
Titel: Conditionally immortalized brown preadipocytes can switch between proliferative and differentiated states
Auteur: Liu J, Kuipers EN, Sips HCM, Dorleijn JC, van Dam AD, Christodoulides C, Karpe F, Zhou G, Boon MR, Rensen PCN, de Vries AAF, Kooijman S
Magazine: Biochimica et Biophysica Acta
Link: https://www.sciencedirect.com/science/article/pii/S1388198119301519?via%3Dihub
Auteur: Liu J, Kuipers EN, Sips HCM, Dorleijn JC, van Dam AD, Christodoulides C, Karpe F, Zhou G, Boon MR, Rensen PCN, de Vries AAF, Kooijman S
Magazine: Biochimica et Biophysica Acta
Link: https://www.sciencedirect.com/science/article/pii/S1388198119301519?via%3Dihub
Titel: Ejection of damaged mitochondria and their removal by macrophages ensure efficient thermogenesis in brown adipose tissue
Auteur: Rosina M, Ceci V, Turchi R, Chuan L, Borcherding N, Sciarretta F, Sánchez-Díaz M, Tortolici F, Karlinsey K, Chiurchiù V, Fuoco C, Giwa R, Field RL, Audano M, Arena S, Palma A, Riccio F, Shamsi F, Renzone G, Verri M, Crescenzi A, Rizza S, Faienza F, Filomeni G, Kooijman S, Rufini S, de Vries AAF, Scaloni A, Mitro N, Tseng YH, Hidalgo A, Zhou B, Brestoff JR, Aquilano K, Lettieri-Barbato D
Magazine: Cell Metabolism
Link: https://www.sciencedirect.com/science/article/pii/S1550413122000882?via%3Dihub
Auteur: Rosina M, Ceci V, Turchi R, Chuan L, Borcherding N, Sciarretta F, Sánchez-Díaz M, Tortolici F, Karlinsey K, Chiurchiù V, Fuoco C, Giwa R, Field RL, Audano M, Arena S, Palma A, Riccio F, Shamsi F, Renzone G, Verri M, Crescenzi A, Rizza S, Faienza F, Filomeni G, Kooijman S, Rufini S, de Vries AAF, Scaloni A, Mitro N, Tseng YH, Hidalgo A, Zhou B, Brestoff JR, Aquilano K, Lettieri-Barbato D
Magazine: Cell Metabolism
Link: https://www.sciencedirect.com/science/article/pii/S1550413122000882?via%3Dihub
Titel: Generation, Characterization, and Application of Inducible Proliferative Adult Human Epicardium-Derived Cells
Auteur: Ge Y, Smits AM, Liu J, Zhang J, van Brakel TJ, Goumans MJTH, Jongbloed MRM, de Vries AAF
Magazine: Cells
Link: https://www.mdpi.com/2073-4409/10/8/2064
Auteur: Ge Y, Smits AM, Liu J, Zhang J, van Brakel TJ, Goumans MJTH, Jongbloed MRM, de Vries AAF
Magazine: Cells
Link: https://www.mdpi.com/2073-4409/10/8/2064
Titel: A physiological glucocorticoid rhythm is an important regulator of brown adipose tissue function
Auteur: Kroon J, Schilperoort M, In Het Panhuis W, van den Berg R, van Doeselaar L, Verzijl CRC, van Trigt N, Mol IM, Sips HHCM, van den Heuvel JK, Koorneef LL, van der Sluis RJ, Fenzl A, Kiefer FW, Vettorazzi S, Tuckermann JP, Biermasz NR, Meijer OC, Rensen PCN, Kooijman S
Magazine: Molecular metabolism
Link: https://www.sciencedirect.com/science/article/pii/S2212877821000193?via%3Dihub
Auteur: Kroon J, Schilperoort M, In Het Panhuis W, van den Berg R, van Doeselaar L, Verzijl CRC, van Trigt N, Mol IM, Sips HHCM, van den Heuvel JK, Koorneef LL, van der Sluis RJ, Fenzl A, Kiefer FW, Vettorazzi S, Tuckermann JP, Biermasz NR, Meijer OC, Rensen PCN, Kooijman S
Magazine: Molecular metabolism
Link: https://www.sciencedirect.com/science/article/pii/S2212877821000193?via%3Dihub
Titel: Engineered 3D vessel-on-chip using hiPSC-derived endothelial- and vascular smooth muscle cells
Auteur: Vila Cuenca M, Cochrane A, van den Hil FE, de Vries AAF, Lesnik Oberstein SAJ, Mummery CL, Orlova VV
Magazine: Stem Cell Reports
Link: https://www.sciencedirect.com/science/article/pii/S2213671121004215?via%3Dihub
Auteur: Vila Cuenca M, Cochrane A, van den Hil FE, de Vries AAF, Lesnik Oberstein SAJ, Mummery CL, Orlova VV
Magazine: Stem Cell Reports
Link: https://www.sciencedirect.com/science/article/pii/S2213671121004215?via%3Dihub
Titel: Generation and primary characterization of iAM-1, a versatile new line of conditionally immortalized atrial myocytes with preserved cardiomyogenic differentiation capacity
Auteur: Liu J, Volkers L, Jangsangthong W, Bart CI, Engels MC, Zhou G, Schalij MJ, Ypey DL, Pijnappels DA, de Vries AAF
Magazine: Cardiovascular Research
Link: https://academic.oup.com/cardiovascres/article/114/14/1848/5039893
Auteur: Liu J, Volkers L, Jangsangthong W, Bart CI, Engels MC, Zhou G, Schalij MJ, Ypey DL, Pijnappels DA, de Vries AAF
Magazine: Cardiovascular Research
Link: https://academic.oup.com/cardiovascres/article/114/14/1848/5039893
Titel: Identification of Functional Variant Enhancers Associated With Atrial Fibrillation
Auteur: van Ouwerkerk AF, Bosada FM, Liu J, Zhang J, van Duijvenboden K, Chaffin M, Tucker NR, Pijnappels D, Ellinor PT, Barnett P, de Vries AAF, Christoffels VM
Magazine: Circulation Research
Link: https://www.ahajournals.org/doi/epub/10.1161/CIRCRESAHA.119.316006
Auteur: van Ouwerkerk AF, Bosada FM, Liu J, Zhang J, van Duijvenboden K, Chaffin M, Tucker NR, Pijnappels D, Ellinor PT, Barnett P, de Vries AAF, Christoffels VM
Magazine: Circulation Research
Link: https://www.ahajournals.org/doi/epub/10.1161/CIRCRESAHA.119.316006
Titel: Self-restoration of cardiac excitation rhythm by anti-arrhythmic ion channel gating
Auteur: Majumder R, De Coster T, Kudryashova N, Verkerk AO, Kazbanov IV, Ördög B, Harlaar N, Wilders R, de Vries AA, Ypey DL, Panfilov AV, Pijnappels DA
Magazine: eLife
Link: https://elifesciences.org/articles/55921
Auteur: Majumder R, De Coster T, Kudryashova N, Verkerk AO, Kazbanov IV, Ördög B, Harlaar N, Wilders R, de Vries AA, Ypey DL, Panfilov AV, Pijnappels DA
Magazine: eLife
Link: https://elifesciences.org/articles/55921
Titel: Androgens modulate glucocorticoid receptor activity in adipose tissue and liver
Auteur: Spaanderman, Dieuwertje C E, Nixon, Mark, Buurstede, Jacobus C, Sips, Hetty H C M, Schilperoort, Maaike, Kuipers, Eline N, Backer, Emma A, Kooijman, Sander, Rensen, Patrick C N, Homer, Natalie Z M, Walker, Brian R, Meijer, Onno C, Kroon, Jan
Magazine: Journal of Endocrinology
Link: https://joe.bioscientifica.com/view/journals/joe/240/1/JOE-18-0503.xml
Auteur: Spaanderman, Dieuwertje C E, Nixon, Mark, Buurstede, Jacobus C, Sips, Hetty H C M, Schilperoort, Maaike, Kuipers, Eline N, Backer, Emma A, Kooijman, Sander, Rensen, Patrick C N, Homer, Natalie Z M, Walker, Brian R, Meijer, Onno C, Kroon, Jan
Magazine: Journal of Endocrinology
Link: https://joe.bioscientifica.com/view/journals/joe/240/1/JOE-18-0503.xml
Titel: Multicellular In vitro Models of Cardiac Arrhythmias: Focus on Atrial Fibrillation
Auteur: van Gorp PRR, Trines SA, Pijnappels DA, de Vries AAF
Magazine: Frontiers in Medicine
Link: https://www.frontiersin.org/articles/10.3389/fcvm.2020.00043/full
Auteur: van Gorp PRR, Trines SA, Pijnappels DA, de Vries AAF
Magazine: Frontiers in Medicine
Link: https://www.frontiersin.org/articles/10.3389/fcvm.2020.00043/full
Titel: Sbk2, a Newly Discovered Atrium-Enriched Regulator of Sarcomere Integrity
Auteur: van Gorp PRR, Zhang J, Liu J, Tsonaka R, Mei H, Dekker SO, Bart CI, De Coster T, Post H, Heck AJR, Schalij MJ, Atsma DE, Pijnappels DA, de Vries AAF
Magazine: Circulation Research
Link: http://10.1161/CIRCRESAHA.121.319300
Auteur: van Gorp PRR, Zhang J, Liu J, Tsonaka R, Mei H, Dekker SO, Bart CI, De Coster T, Post H, Heck AJR, Schalij MJ, Atsma DE, Pijnappels DA, de Vries AAF
Magazine: Circulation Research
Link: http://10.1161/CIRCRESAHA.121.319300
Titel: Androgens modulate glucocorticoid receptor activity in adipose tissue and liver
Auteur: Spaanderman DCE, Nixon M, Buurstede JC, Sips HC, Schilperoort M, Kuipers EN, Backer EA, Kooijman S, Rensen PCN, Homer NZM, Walker BR, Meijer OC, Kroon J
Magazine: Journal of Endocrinology
Auteur: Spaanderman DCE, Nixon M, Buurstede JC, Sips HC, Schilperoort M, Kuipers EN, Backer EA, Kooijman S, Rensen PCN, Homer NZM, Walker BR, Meijer OC, Kroon J
Magazine: Journal of Endocrinology
Titel: Identification of atrial fibrillation associated genes and functional non-coding variants
Auteur: van Ouwerkerk AF, Bosada FM, van Duijvenboden K, Hill MC, Montefiori LE, Scholman KT, Liu J, de Vries AAF, Boukens BJ, Ellinor PT, Goumans MJTH, Efimov IR, Nobrega MA, Barnett P, Martin JF, Christoffels VM
Magazine: Nature Communications
Link: https://www.nature.com/articles/s41467-019-12721-5
Auteur: van Ouwerkerk AF, Bosada FM, van Duijvenboden K, Hill MC, Montefiori LE, Scholman KT, Liu J, de Vries AAF, Boukens BJ, Ellinor PT, Goumans MJTH, Efimov IR, Nobrega MA, Barnett P, Martin JF, Christoffels VM
Magazine: Nature Communications
Link: https://www.nature.com/articles/s41467-019-12721-5
Titel: Selective Glucocorticoid Receptor Antagonist CORT125281 Activates Brown Adipose Tissue and Alters Lipid Distribution in Male Mice
Auteur: Kroon, Jan, Koorneef, Lisa L, van den Heuvel, Jose K, Verzijl, Cristy R C, van de Velde, Nienke M, Mol, Isabel M, Sips, Hetty C M, Hunt, Hazel, Rensen, Patrick C N, Meijer, Onno C
Magazine: Endocrinology
Link: https://academic.oup.com/endo/article/159/1/535/4159870
Auteur: Kroon, Jan, Koorneef, Lisa L, van den Heuvel, Jose K, Verzijl, Cristy R C, van de Velde, Nienke M, Mol, Isabel M, Sips, Hetty C M, Hunt, Hazel, Rensen, Patrick C N, Meijer, Onno C
Magazine: Endocrinology
Link: https://academic.oup.com/endo/article/159/1/535/4159870
Titel: Conditional immortalization of human atrial myocytes for the generation of in vitro models of atrial fibrillation
Auteur: Harlaar N, Dekker SO, Zhang J, Snabel RR, Veldkamp MW, Verkerk AO, Fabres CC, Schwach V, Lerink LJS, Rivaud MR, Mulder AA, Corver WE, Goumans MJTH, Dobrev D, Klautz RJM, Schalij MJ, Veenstra GJC, Passier R, van Brakel TJ, Pijnappels DA, de Vries AAF
Magazine: Nature Biomedical Engineering
Link: https://www.nature.com/articles/s41551-021-00827-5
Auteur: Harlaar N, Dekker SO, Zhang J, Snabel RR, Veldkamp MW, Verkerk AO, Fabres CC, Schwach V, Lerink LJS, Rivaud MR, Mulder AA, Corver WE, Goumans MJTH, Dobrev D, Klautz RJM, Schalij MJ, Veenstra GJC, Passier R, van Brakel TJ, Pijnappels DA, de Vries AAF
Magazine: Nature Biomedical Engineering
Link: https://www.nature.com/articles/s41551-021-00827-5
Titel: Conditionally immortalized cells and methods for their preparation
Auteur: Antonius Adrianus Franciscus DE VRIES, Daniël Antonie PIJNAPPELS, Martin Jan SCHALY
Link: https://patents.google.com/patent/WO2018135948A1/en
Auteur: Antonius Adrianus Franciscus DE VRIES, Daniël Antonie PIJNAPPELS, Martin Jan SCHALY
Link: https://patents.google.com/patent/WO2018135948A1/en
Titel: Modelling of atrial fibrillation at physiologically relevant scales enabled by massive expansion of native human atrial cardiomyocytes